Table 1 List of primers used for plasmodium species identification using nested real-time PCR
From: Molecular surveillance and temporal monitoring of malaria parasites in focal Vietnamese provinces
| Genus/species | Target | Primer ID | Primer sequence (5′ – 3′) | 5′ modified | 3′ modified | References |
|---|---|---|---|---|---|---|
| Plasmodium | 18S rRNA gene | rPLU6-F | TTAAAATTGTTGCAGTTAAAACG | [21] | ||
| rPLU5-R | CCTGTTGTTGCCTTAAACTTC | |||||
| P. falciparum | 18S rRNA type S | PF-F | ATTGCTTTTGAGAGGTTTTGTTACTTT | [22] | ||
| PF-R | GCTGTAGTATTCAAACACAATGAACTCAA | |||||
| PF-Probe | CATAACAGACGGGTAGTCAT | HEX | MGBEQ | |||
| P. vivax | 18S rRNA type A | VIV-F | GCAACGCTTCTAGCTTAATCCAC | [23] | ||
| VIV-R | CAAGCCGAAGCAAAGAAAGTCC | |||||
| VIV-Probe | ACTTTGTGCGCATTTTGCTA | HEX | MGBEQ | |||
| P. malariae | 18S rRNA gene | PM-F | GGTGTTGGATGATAGAGTAA | [20] | ||
| PM-R | CCCAAAGACTTTGATTTCTC | |||||
| PM-Probe | AGGAAGCTATCTAAAAGAAACACTCAT | HEX | BHQ-1 | |||
| P. ovale curtisi | 18S rRNA gene | POS-F | ATTTCAAAGAGTCATGGCGTTTCTG | [20] | ||
| POS-R | TTGTAAAGGAGACACTTTCTTGAAATCG | |||||
| POS-Probe | CTCCTTGGTCGATCTGCCCAGCACT | FAM | BHQ-1 | [20] | ||
| P. ovale wallikeri | 18S rRNA gene | POS-F | ATTTCAAAGAGTCATGGCGTTTCTG | |||
| POW-R | TGTAAAGGAGACAACTTTCTTGGAGCTA | |||||
| POW-Probe | TTGATCGCCCAGCACTGACCATCT | HEX | BHQ-1 |